normal prostate epithelial cell line pnt1a  (European Collection of Authenticated Cell Cultures)

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: The secretion of testosterone ( a , b ), estradiol ( c , d ), and progesterone ( e , f ) after 48 h of exposure to AOH and DHEA, evaluated by ELISA tests in PC3 and PNT1A cells. Results are presented as mean ± SE. One-way ANOVA was used to perform statistical analysis of the results, and p < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of CAV-1. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, CAV-1—caveolin 1.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce apoptosis after 48 h of exposure in PC3 and PNT1A cells ( a ). Analysis of apoptotic cells based on flow cytometry with representative results ( b ). One-way ANOVA was used for statistical analysis. Results are presented and mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001, as compared to the control. Representative results of Western blot analysis of CASP3 ( c ). Analysis of the relative expression of ANX5a ( d ) and TP53 genes in normal and prostate cancer cell lines ( e ). AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Flow Cytometry, Control, Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of Casp3 and Cleaved Casp3. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce changes in cell cycle progression after 48 h of exposure in PNT1A ( a ) and PC3 cells ( b ). Analysis based on the results obtained in flow cytometry. Analysis of the relative expression of CDKN1A ( c ) and CDK2 genes in normal and prostate cancer cell lines ( d ). One-way ANOVA was used for statistical analysis. The results are presented as mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control. The results were acquired in three independent experiments.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Flow Cytometry, Expressing, Control

Journal: Oncogenesis

Article Title: Dihydroartemisinin inhibits prostate cancer via JARID2/miR-7/miR-34a-dependent downregulation of Axl

doi: 10.1038/s41389-019-0122-6

Figure Lengend Snippet: Cells were treated with DHA (5 μM) or DMSO (0.05%) for 24 h. a Heat map of miRNA microarray expression data. Cluster analysis classified the samples into groups based on miRNA expression levels in each sample. Red indicates high expression and green indicates relatively low expression of miRNA. b Schematic representation of the location of the putative miR-7 target site is shown in the Axl 3′-UTR. c RT-PCR analysis of miR-34 expression levels (left) and miR-7 (right) expression levels in PCa cell lines. ΔCt values graphed are relative to the endogenous control RNU6B small RNA and data were normalized to normal cell PNT1A. Data are representative of three independent experiments and all values shown are mean ± SEM from a representative experiment; * p < 0.05, two-tailed Student’s t- test. d RT-PCR analysis of expression levels of miR-34a, miR-7, and Axl in human PCa samples. Total RNA was collected from human tissue consisting of paired normal and tumor samples and was analyzed for miR-7, miR-34a, and Axl expression levels. ΔCt values graphed are relative to the endogenous control RNU6B small RNA (for miR-34a and miR-7) and GAPDH for Axl. Data are shown as the triplicate independent experiments; * p < 0.05, two-tailed, nonparametric Mann–Whitney test. e RT-PCR analysis of expression levels of miR-34a (right), miR-7 (left) and Axl (bottom) in tumor extracted from mice treated with 40 mg/kg of DHA or DMSO (2 mL/kg). Total RNA was collected from tumor and analyzed for miR-7 and miR-34a expression levels. ΔCt values graphed are relative to the endogenous control RNU6B small RNA. Data are representative of three independent experiments and all values shown are mean ± SD from a representative experiment; * p < 0.05, two-tailed, nonparametric Mann–Whitney test

Article Snippet: PNT1A, a normal prostate epithelial cell line, was purchased from Sigma Aldrich.

Techniques: Microarray, Expressing, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test, MANN-WHITNEY